rabbit anti rat cd133 antibody  (Novus Biologicals)

Journal: World Journal of Gastroenterology

Article Title: Transplantation of bone marrow-derived endothelial progenitor cells and hepatocyte stem cells from liver fibrosis rats ameliorates liver fibrosis

doi: 10.3748/wjg.v24.i2.237

Figure Lengend Snippet: Phagocytosis and vasculogenesis functions of BM-EPCs of liver fibrosis rats. A: Bone marrow-derived endothelial progenitor cells uptake of Dil-ac-LDL (red); B: BM-EPCs binding with FITC-UEA-1 (green); C: Merge of A and B (yellow); D: Vascular network-like structures of BM-EPCs on Matrigel. Bars: 50 μm (A-C); 200 μm (D); BM-EPCs: Bone marrow-derived endothelial progenitor cells.

Article Snippet: After 10 d of culture under inducing conditions, 2 × 10 6 BM-EPCs were incubated with the FcR blocking reagent (Miltenyi Biotec Inc., Auburn, CA, United States) and fluorescein isothiocyanate (FITC)-conjugated rabbit anti-rat CD133 antibody (BD Biosciences) and rabbit anti-rat vascular endothelial growth factor receptor 2 (VEGFR2) antibody (BD Biosciences) for 30 min at 4 °C, respectively.

Techniques: Derivative Assay, Binding Assay

Journal: Cardiovascular Diagnosis and Therapy

Article Title: A new method of culturing rat bone marrow endothelial progenitor cells in vitro

doi: 10.21037/cdt-20-536

Figure Lengend Snippet: Immunocytochemical analysis of BMMNCs. After 14 days of culture, BMMNCs revealed expression of rat stem cell markers (×400). (A) CD34 is positive and the cytoplasm is brown (86.36±5.12); (B) CD45 is positive (30.25±4.62); (C) CD133 is positive (19.72±5.20).

Article Snippet: We used primary rabbit polyclonal antibodies against rat CD133 (BioTech, Beijing, China) and rat VEGFR2 (Boster, Wuhan, China).

Techniques: Expressing

Journal: Cardiovascular Diagnosis and Therapy

Article Title: A new method of culturing rat bone marrow endothelial progenitor cells in vitro

doi: 10.21037/cdt-20-536

Figure Lengend Snippet: Flow cytometry analysis of BMMNCs differentiating into EPCs. After 12 days in vitro, the results of flow cytometry show that: (A) CD34 is positive (89.0%); (B) CD45 is positive (83.2%); (C) CD133 is positive (75.5%). BMMNCs, bone-marrow mononuclear cells; EPCs, endothelial progenitor cells.

Article Snippet: We used primary rabbit polyclonal antibodies against rat CD133 (BioTech, Beijing, China) and rat VEGFR2 (Boster, Wuhan, China).

Techniques: Flow Cytometry, In Vitro

Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response

doi: 10.1631/jzus.b1600500

Figure Lengend Snippet: Fig. 2 Representative photomicrographs of immunohistochemical staining Adjacent sections of lung tissues showing the expressions of CD133, VEGFR-2 as well as HGF in a normal pulmonary arteriole (a‒c), an immature plexiform lesion (PL) (d‒f), and a mature lesion (g‒i). The lung sections presented here are from 2-week-old broilers. Mɸ: foam-like macrophage. Scale bar=25 μm

Article Snippet: After blocking with 5% (0.05 g/ml) bovine serum albumin, the slides were incubated with the following antibodies: polyclone rabbit anti-rat CD133 (1:50 (v/v), Bostar Biological Technology, China), VEGFR-2 (1:50 (v/v), Boster Biotechnology Technology, China), and HGF (1:50 (v/v), Bostar Biological Technology, China).

Techniques: Immunohistochemical staining, Staining

Journal: Journal of Zhejiang University-SCIENCE B

Article Title: Involvement of endothelial progenitor cells in the formation of plexiform lesions in broiler chickens: possible role of local immune/inflammatory response

doi: 10.1631/jzus.b1600500

Figure Lengend Snippet: Fig. 3 Characterization of endothelial progenitor cells (EPCs) EPCs displayed a spindle-shape appearance on Day 7 of plating (a) and differentiated into monolayer of cobblestone-like cells on Day 14 (b). The ability of EPCs for DiL-ac-LDL uptake (red color) and the binding of FITC-UEA-1 lectin (green color) are shown in (c), while cells carrying both characteristics appear in orange. (d) Expressions of CD133 and VEGFR-2 were assessed by flow cytometry (gray line indicates the isotype control; black line indicates the antibody staining). Representative results are shown. Scale bar= 100 μm (Note: for interpretation of the references to color in this figure legend, the reader is referred to the web version of this article)

Article Snippet: After blocking with 5% (0.05 g/ml) bovine serum albumin, the slides were incubated with the following antibodies: polyclone rabbit anti-rat CD133 (1:50 (v/v), Bostar Biological Technology, China), VEGFR-2 (1:50 (v/v), Boster Biotechnology Technology, China), and HGF (1:50 (v/v), Bostar Biological Technology, China).

Techniques: Binding Assay, Flow Cytometry, Control, Staining